Reagent CK-MB

100 TESTS – 04525299190. Reagent for Biochemical Analyzer Roche Cobas Integra 400 & Cobas Integra 400+

Summary
In vitro test for the quantitative determination of the catalytic activity of creatine kinase MB subunit (CK‑MB) in human serum and plasma on COBAS INTEGRA systems. Immunological UV assay
▪ Sample and addition of R1 (buffer/enzymes/coenzyme)
▪ Addition of R2 (buffer/substrate/antibody) and start of reaction.
Human CK‑MB is composed of two subunits, CK‑M and CK‑B which both have an active site. With the aid of specific antibodies to CK‑M, the catalytic activity of CK‑M subunits in the sample is inhibited to 99.6 % without affecting the CK‑B subunits. The remaining CK‑B activity, corresponding to
half the CK‑MB activity, is determined by the total CK method. As the CK‑BB isoenzyme only rarely appears in serum and the catalytic activity of the CK‑M and CK‑B subunits hardly differ, the catalytic activity of the CK‑MB isoenzyme can be calculated from the measured CK‑B activity by multiplying the result by 2.

Combine Reagent CK-MB with Calset or Precicontrol shown below.